xGen Lockdown Probes are compatible with the NimbleGen SeqCap EZ Reagent Kit (Roche) and the SureSelect Reagent Kit* (Agilent). Dont let up. 100 bp, paired-end sequencing was performed on an Illumina HiSeq 2500 instrument. Read user reviews, compare products & request pricing from manufacturers of NGS Preparation Kits | Next Generation Sequencing The xGen Hybridization and Wash Kit has been developed and optimized for use with xGen Lockdown Probes and Panels and xGen Blocking Oligos. The xGen Hybridization Vortex and briefly spin down. This patent application was filed with the USPTO on Wednesday, February 26, 2020 ThruPLEX library capture. xGen Lockdown Panels can be customized by supplementing with additional probes. This opens in a new window. xGen NGSmade for you. This video provides a quick introduction to the design of custom target enrichment probes using IDT's xGen Lockdown Design Tool. SARS-CoV-2. Your product is now available from Integrated DNA Technologies. With which sequencing protocols are xGen Hyb Panels compatible? xGen Lockdown Panels can be customized by supplementing with additional probes. * SureSelect reagents are not recommended for targeting genomic regions with >70% A/T. Custom xGen Blocking Oligos. Pre-formulated mixes for ligation-based library prep or Nextera library prep kits. Published on: October 17, 2017. The xGen Hybridization and Wash Kit was used according to the protocol in xGen hybridization capture of DNA libraries for NGS target enrichment. For plasma cfDNA library, we used a customized DNA probes in 150 genes (GP150) with unique IDT bioinformatics scientists have created the xGen Off-Target QC Method that uses a sequence identity-based approach to screen your custom hybridization probes. xGen Lockdown Probe Pools | IDT Expand the content of existing panels, deepen coverage for monitoring rare alleles, and rescue regions not well-covered by other capture methods. This method can expand the size of your target space without increasing the risk of off-target capture (Figure 1). The xGen Prism DNA Library Prep Kit delivers higher conversion rates, complexity and coverage. xGen Lockdown Probes Kit (Integrated DNA Technologies), and thereafter IDT xGen Human Exome Research Panel kit was used to capture targeted DNAs for FFPE gDNA following the standard protocol. Lotus DNA Library Prep Kit Protocol (2.4 MB) Quick reference protocol: Lotus DNA Library Prep Kit PCR-amplified (1.1 MB) Quick reference protocol: xGen Prism DNA Library Prep Kit (1.2 MB) Quick reference protocol: Lotus DNA Library Prep Kit Targeted sequencing (1015 KB) To enrich for mRNAs, target enrichment was performed using xGen Lockdown Reagents from Integrated DNA Technologies (IDT, Cat# 1072281) designed to capture the entire exome . Following the DNA denaturation step at 95C, immediately add 4L of the Lockdown pool of probes to the tube (3-4pmol of probes). In brief, genomic DNA was prepared with a cus- To identify novel risk genes, we performed a gene-based tomized reagent kit from Kapa Biosystems and captured case-control association test comparing the frequency of using Integrated DNA Technologies xGen lockdown rare deleterious variants in PAH cases with population probes. Figure 3. Automated runs were performed using the Perkin Elmer Sciclone Workstation with automation scripts developed jointly by IDT and Perkin Elmer. SYSTEMS AND METHODS FOR USING SEQUENCING DATA FOR PATHOGEN DETECTION is an invention by Ariane Lozac'hmeur, Chicago IL UNITED STATES. Custom blocking oligos can be ordered for specialized applications or other sequencing platforms, and are offered with specified modifications. Resuspend xGen Panel to 4.5 l per hybridization in nuclease-free water and aliquot for single use into 0.2-ml tubes. Read user reviews, compare products & request pricing from manufacturers of In-Situ Hybridization | Gene Expression and Molecular Cloning The distribution deal bolsters the partnership between IDT and Beckman Coulter Life Science to make high-performance NGS solutions more accessible to fast-growing labs seeking to generate libraries more efficiently with out-of-the-box automation. XGENCAP: 95C, 10 minutes 65C, Heated lid set to 750C 4.3. Expand the content of existing panels, deepen coverage for monitoring rare alleles, and rescue regions not well-covered by other capture methods. Unsure of what products are available? xGen NGS for whole exome sequencing (WES) includes offerings for routine and difficult samples, minimizing efforts while maintaining key data metrics and positioning your research. Your product is now available from Integrated DNA Technologies. Final volume should be 15 L. Please contact your NGS Sales Representative or applicationsupport@idtdna.com for more information on IDTs full NGS workflow. xGen Universal Blockers are offered in 16-, 96-, and 4 x 96-reaction formats for convenient experiment design and for use with xGen Lockdown Panels or xGen Lockdown Probe sets. Transfer resuspended material to a 0.2 mL PCR tube and incubate in a thermal cycler at 95C for 10 min. Libraries were generated according to the manufacturers instructions with 10 ng of cfDNA reference standards, then captured with a custom 61 kb xGen Lockdown Panel. IDT offers multiple library prep kits that are compatible with the xGen NGS Hybridization Capture workflow. The IDT xGen hybridization capture products includes a variety of predesigned panels and custom panels available in a range of panel sizes. It also broadens the suite of automation tools and solutions IDT is able to offer its customers. Your product is now available from Integrated DNA Technologies. Figure 1. Welcome to the IDT family! Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen NGS. Vortex and briefly spin down. Legacy protocolslibrary prep. Limit non-specific binding during in-solution hybridization target capture. You can contact xGen@idtdna.com to obtain the protocol or inquire about kit compatibility. Resuspend xGen Universal Blocking Oligos to 1 l per reaction (or 1 nmol/l) in nuclease-free water. e. Add 2 L Lockdown Probe pool (3 pmol total) to the tube. Well help. The xGen Off-Target QC Method expands target region. CORALVILLE, Iowa & INDIANAPOLIS, June 07, 2022--Integrated DNA Technologies and Beckman Coulter Life Sciences have entered into a new agreement, broadening IDT's suite of automation tools, solutions Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions. Add the probes directly to the tube in the thermal cycler (final volume = 17L). The kit is compatible with the protocol, xGen hybridization capture of DNA libraries for NGS target enrichment, v1, to provide a complete, high-quality hybridization capture solution for targeted NGS. An updated, automation-friendly protocol is available for high-throughput applications. The xGen Hyb Panel Design Tool can help guide you through the process of designing a panel specific for your research. f. Incubate hybridization reaction